PDestTol2CG*: Difference between revisions
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Hggenusrwki (talk | contribs) Created page with "== Construction Details == This template is used to display DNA sequence information in a standardized format. == Crude Map == photo showing locations of components == Sequence == * Annotated Sequence: (Genbank format) link * Full-Length Sequence with individual component sequences included: (FASTA file) link == Reference == This template is used to display DNA sequence information in a standardized format." |
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== Construction Details == | == Construction Details == | ||
pDestTol2CG was made by PCRing the cmlc2:egfp-pA cassette, adding BglII sites in the primers, then cloning into the unique BglII site of pDestTol2pA and selecting for reverse orientation relative to the gate and existing polyA signal. The cmlc2:egfp cassette has not been sequenced, but works in functional tests. | |||
== Crudely Annotated Sequence == | |||
FASTA file with the full-length sequence as well as sequences of individual components: | |||
: pDestTol2CG sequence | |||
Edited 7 June 2007 to add six bases (a KpnI site that was missing). | |||
== Crude Map == | == Crude Map == | ||
Screenshot from Sequencher showing locations of components: | |||
PDestTol2CG.png | |||
Latest revision as of 16:49, 13 March 2025
Construction Details
pDestTol2CG was made by PCRing the cmlc2:egfp-pA cassette, adding BglII sites in the primers, then cloning into the unique BglII site of pDestTol2pA and selecting for reverse orientation relative to the gate and existing polyA signal. The cmlc2:egfp cassette has not been sequenced, but works in functional tests.
Crudely Annotated Sequence
FASTA file with the full-length sequence as well as sequences of individual components:
- pDestTol2CG sequence
Edited 7 June 2007 to add six bases (a KpnI site that was missing).
Crude Map
Screenshot from Sequencher showing locations of components: PDestTol2CG.png