View source for PME-EGFP no stop

== Construction Details ==
pME-EGFP no stop was made by PCR amplification of the EGFP insert from a pCS2-based plasmid, using primers that add att sites, followed by a BP reaction. Sequencing confirms that all bases of the insert are correct.

This clone can be used to generate N-terminal EGFP fusions with a gene of interest placed in a 3' clone.

== Crude Map ==
Screenshot from ApE showing locations of components:

:[[File:455 pME-EGFPnostopcrop.PNG|thumb|none|350px|455 pME-EGFP no stop]]

==Download the Sequence ==
* '''Annotated Sequence'''(Genbank format) and '''Full-Length Sequence with individual component sequences included'''(FASTA file)
: [[Media:455.pME-EGFP no stop.zip|Download pME-EGFP no stop]]