View source for P5E-MCS

== Construction Details ==
p5E-MCS was made by PCR of the pBSII SK+ multiple cloning site, from T7 to T3 primers, followed by a BP reaction with pDONR P4-P1R. Sequencing confirms that all bases of the insert are correct.

M13F, M13R, and T3 should all work as sequencing primers.

Unfortunately, the backbone of P4-P1R contains recognition sites for several potentially useful restriction enzymes. The restriction sites from the Bluescript MCS that remain unique in p5E-MCS are:

''Asp718-KpnI-DraII-XhoI-SalI-Bsp106-HindIII-SmaI-BamHI-SpeI-SacII-BstXI''

== Crude Map ==
Screenshot from ApE showing locations of components:

:[[File:228 p5E-MCS.PNG|thumb|none|350px|228 p5E-MCS]]

==Download the Sequence ==
* '''Annotated Sequence'''(Genbank format) and '''Full-Length Sequence with individual component sequences included'''(FASTA file)
: [[Media:228.p5E-MCS.zip|Download p5E-MCS]]